Oregon State University

Doubled Haploid Production

Why doubled haploids?  Breeding self-pollinated crops is a long-term process that requires multiple cycles of self-pollination to achieve complete homozygosity. Varietal purity and efficient molecular breeding require complete homozygosity. Doubled haploid (DH) production leads to complete homozygosity in a single generation, thus bypassing the complications of field, greenhouse, or off-season generation advance. The barley breeding program at Oregon State University started a doubled haploid production facility in 2011. After three years of contract production, we have decided to focus the lab on our own breeding program, on specific grant-funded research, and research collaborations.

If you are interested in developing a grant-funded project, or a research collaboration, please contact Pat Hayes.

Our DH production system is based on anther culture: We regenerate green plantlets from microspores through the formation of embryoids that subsequently germinate to produce doubled haploid plants. We obtain an average of 14 fertile DH plants per F1 donor plant, with a range of 2 – 40 DH/F1. The spontaneous chromosome doubling rate is > 80%. The process - from planting F1 seed to the harvest of DH seed - takes approximately 9 months for spring genotypes and 12 months for winter genotypes. Please click here for a poster illustrating the process (and project members).

 


OSU DH facility staff:

  • Tanya Filichkin. Tissue Culture Lab
  • Laura Helgerson. Greenhouse Production
  • Josh Daker. Student worker



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