Naked Eye Polymorphisms (NEPs) segregating in the OWBs

Dominant	Recessive	Chromosome location
Blp: black head	blp: white head	1H	Blp Black head; *blp* non-black. The black allele here is strong. However some difficulty may be experienced in determining its presence in combination with Pre2 and the orange lemma, rob.
Cer-yy: Shiny head	Cer-yy: non-shiny head	1H	Dr. Jerry Franckowiak identified Cer-yy, a dominant shiny head, in the OWB-D. Scoring the recessive allele may be challenging.
Pre2: purple lemma and pericarp	pre2: non-purple lemma and pericarp	2H	*Pre2* Purple lemma and pericarp; *pre2* non-purple lemma and pericarp In black lines Pre2 is visible only just prior to development of black pigment. Check for it regularly where you only have one or two heads per plant.
Vrs1: 2-row	vrs1: 6-row	2H	Vrs1 Two-row; vrs1 six-row. The lateral six-row seeds tend to be somewhat smaller than those found in six-row barley, and seed set is lower. Under sub optimal growing conditions, lateral seed set may be poor - leading to difficulties in scoring this trait.
Wst: no stripes on leaves	wst: white stripes on seedling leaves	2H	Wst Normal green leaves; wst white stripes on some of the seedling leaves. This trait shows incomplete penetrance. Most plants homozygous for the recessive can be determined. The stripe is most easily visible under cool growing conditions.
Zeo: dwarf with dense head	zeo: normal height	2H	Zeo Semi-dominant dwarf with dense head; zeo normal height. The Zeo Zeo head is very short. The distance between nodes in the head is reduced. There is a tendency for some of the glumes to be enlarged.
Alm: normal green lemma and nodes	alm: albino lemma and nodes	3H	Alm Normal green color of lemma and nodes; alm albino lemma and nodes. The recessive allele conditions a loss of green color in the central part of the lemma. The nodes also appear white. Score before masked by Blp, and before ripening and loss of green color occurs.
Btr1: brittle rachis	btr1: tough rachis	3H	Btr1 Wild or brittle rachis; btr1 tough domesticated rachis. The top part of the normal-length brittle rachis head breaks (disarticulates) at maturity. One internode of the rachis remains with each seed in two- row barley, and with each group of three seeds in six- row. This is because the abscission layers in the rachis are just above the points of attachment of the seed. In domesticated barley the development of this abscission layer is suppressed, and upon threshing the break generally occurs at the base of the seed. When one forces a separation at the abscission layer on the rachis of a normal-length ripe head, the break-point is smooth when the Btr1 allele is present, and somewhat ragged when btr1 or btr2, are present.
Pub: pubescence on upper leaf blades	pub: lack of pubescence	3H	Pub Pubescence on upper leaf blades; pub lack of same. The hairs are very short, and give a slightly rough feel to the leaf. Misting the leaves and use of a good light will aid in scoring this trait.
Hsh: hairs on lower leaf sheaths	hsh: lack of hairs on lower leaf sheaths	4H	Hsh Hairs on lower leaf sheaths; hsh lack of hairs.
Kap: hooded	kap: awn	4H	*Kap* Hood; *kap* awn The hood is a misplaced, usually vestigial, floret. The hood is not observed in the short awned (lks2 lks2) homozygotes.
Srh: long hairs on rachilla	srh: short hairs	5H	Srh Long hairs on rachilla; *srh* short hairs on rachilla. The two alleles represented in the OWB may be the only two in the species, although there seem to be modifiers for hair length. The short haired allele has hairs up to about a length equal to the width of the rachilla. The long haired allele has hairs considerably longer than this.
Raw1: rough awn	raw1: smooth awn	5H	Raw1 Rough awn; raw1 awn smooth at base on edges. In the smooth awned plant the tip of the awn is usually rough. In some plants carrying the smooth awned allele the roughness can extend most of the way down the awn. This smooth awned allele is also pleiotropic for reduced branching of stigma. The barbing on the mid-rib of the awn seems to be controlled by one or more other genes. Barbing or lack of it may also show up at the base of the hood. The barbing effect is produced by small almost microscopic thorns.
Rob: normal green lemma and nodes	rob: orange lemma and nodes	6H	Rob Normal green lemma and nodes; rob orange lemma and nodes. The orange lemma gives an orange cast to the lower part of the lemma, both when it is green and on non-black ripe seed. Orange lemma can generally be detected on ripe heads carrying the black allele. The orange coloration also tends to show up on stem nodes. The orange lemma in combination with the albino lemma, alm, can be quite beautiful in immature heads.
Nud: Hulled	nud: hulless	7H	Nud Hulled; nud hulless. The hulless phenotype lacks glue between the seed and the lemma and palea (the hulls). Therefore, the seed threshes clean.
Wax: Non-Waxy starch (Blue stain)	wax: waxy starch (purple stain)	7H	Wax Blue stain of seed starch with dilute KI solution; wax purple stain of seed starch. Adjust KI concentration with water to control speed of color change. Toenail clippers work well for cutting off small amounts of endosperm. Tincture of iodine works well, and can be bought in drug stores.