Resistance to barley stripe rust

Thanks to collaboration with the USDA-ARS (with funding provided by a NACA) we are working with colleagues at ARS, Aberdeen, ID; UC Davis, CA; and ARS-Pullman, WA to characterize levels of adult plant resistance to barley stripe rust, identify the QTLs/genes responsible for the resistance phenotype, and to deploy resistance alleles in new genetic backgrounds.

The following hyperlinks will take you to a chronological listing of reports and publications on various areas of endeavor related to barley stripe rust. To access the data underlying the reports, please send a request*  for access to the Box folder containing a treasure trove of phenotype and genotype data. Dr. Xianming Chen (USDA-ARS) conducts essential and comprehensive stripe rust assessments on a range of germplasm, including some of the experiments described on this page.

BSR screening trial (BSRST). Advanced lines/varieties and checks submitted by OSU, WSU, UC-Davis, and USDA-ARS (ID). Phenotype data: annual. Corvallis, OR and Davis, CA. Genotype data: NA.

BSR validation. A BSR-resistant germplasm array of 55 doubled haploid lines selected from Cycle I and II. 4 checks. Phenotype data: 2020; Barley Stripe Rust and Scald. Corvallis, OR and Davis, CA. Genotype data: In process.

Cycle 0: A germplasm array of 135 doubled haploid advanced lines/varieties. 4 checks. Phenotype data: 2016; Barley Stripe Rust. Corvallis, OR and Davis, CA. Genotype data: Illumina 9K.

Cycle I: A germplasm array of 117 doubled haploid lines derived from crosses among 13 parents. 3 checks. Phenotype data: 2016 and 2017; Barley Stripe Rust and Leaf Rust. Corvallis, OR and  Davis, CA. Genotype data: Illumina 9K + allele-specific primers Rpg1, Rpg5.

Cycle II: A germplasm array of 358 doubled haploid lines derived from crosses among 13 parents. 3 checks. Phenotype data: 2018 and 2019; Barley Stripe Rust, Leaf Rust, and Scald. Corvallis, OR and  Davis, CA. Genotype data: Illumina 50K + allele-specific primers Rpg5.

Cycle III:  An array of 373 doubled haploids was generated from crosses among OSU germplasm that exhibited resistance to race TTKSK at the seedling stage. None of this germplasm amplified for rpg4/Rpg5. These are, therefore, potential new sources of resistance to TTKSK. These resistance donors were crossed with winter/facultative malting and winter/facultative malting barley germplasm from OSU Barley Breeding Program. The array was phenotyped for stripe rust and scald under field conditions at Corvallis, OR (2 years) and Davis, CA (1 year). Stem rust at the seedling stage was assessed under controlled environment conditions at University of Minnesota. In addition, the array was phenotyped for vernalization sensitivity under field conditions at Corvallis, OR in the spring of 2021 to validate allele-specific KASP assays for vernalization and photoperiod sensitivity genes. The array was genotyped at the USDA-ARS Fargo Genotyping Lab using using the Illumina 50K SNP platform.

  • Report
  • Publication (in progress)
     

Cycle IV: An array of 661 genotypes (primarily doubled haploids and their parents) was assembled for phenotyping and genotyping. The array consists of germplasm from five sources within the OSU barley program (1) elite malting 2-row winter and facultative types with resistance to multiple diseases and potential for brewing and distilling end-uses; (2) 2-row winter and facultative selections developed for the US Wheat and Barley Scab Initiative with potential malting quality; (3) 2-row and 6-row winter and facultative selections from the USDA-OREI fall naked diversity panel with potential multi-purpose end uses; (4) 2-row winter and facultative selections from the AMBA LTT panel; (5) 2-row and 6-row winter selections from Cycle 2; (5) 2-row winter, facultative, and spring selections from Cycles 1 and 2.  Selected subsets were phenotyped at Bozeman, MT, and Pullman, WA. Some of the germplasm in Cycle 4 has already been genotyped with the Illumina 50K. The remainder of the germplasm will be genotyped with the same platform (or a new SNP platform under development at the USDA-ARS Fargo Lab) in 2022. Phenotypic data was generated on vernalization sensitivity using a spring-planted field trial at Corvallis, OR and to prevailing disease (notably stripe rust and scald) at Corvallis, OR and Davis, CA. Malting quality will be assessed on a subsample of lines from the Corvallis, Bozeman, and Pullman trials.

  • Report
  • Publication (in progress)

Cycle V: An array of 267 genotypes (primarily doubled haploids and their parents) was assembled for phenotyping and genotyping. The array consists of germplasm from two sources within the OSU barley program (1) elite malting 2-row winter and facultative types with resistance to multiple diseases and potential for brewing and distilling end-uses; (2) naked 2-row winter and facultative naked selections with potential for malting and downstream uses. The array will be phenotyped for prevailing diseases under fall-planted field conditions at Corvallis, OR and Davis, CA; for low temperature tolerance at Lincoln, NE; and for vernalization sensitivity under spring-planted conditions at Corvallis, OR. Samples from the 2021 crop at Corvallis (also the seed source for Cycle 5) will be the target of a collaborative effort to map genes determining malting quality and water sensitivity (a key climate change-related trait) with the Hartwick Center for Craft Food and Beverage. The array will be genotyped with the new SNP platform under development at the USDA-ARS lab at Fargo, ND.

 

*Please send requests for data access, and any questions/suggestions to franciscojavier.hernandezvasquez@oregonstate.edu